ORFprimer Primer design made easy via this Java tool
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ORFprimer Description

ORFprimer is a Java-based software specially created for automatical primer design. It is based on our previously released software ORFer that reads the GenBank XML- and fasta- sequence format. Protein sequences are typically downloaded by simply specifying the GI identifier or accession number of the corresponding protein target. ORFer will extract the open reading frame from the requested protein sequences and display it. ORFprimer automatically calculates PCR primers for these sequences. The handling of ORFprimer is intuitional and quite simple using the default settings. In addition, it provides many possibilities to change the calculation options before and also after the computation of primers, such as: · shift of the primers position, · change of the desired melting temperature, · change of the restriction sites in the primers sequences. This way ORFprimer combines convenience and adaptability in one program which makes it a powerful software tool.

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